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目的:考察家兔血浆中甲基苯丙胺和氯胺酮同时衍生化后GC/MS检测分析结果。方法:血浆样品中加入内标物丙基解痉素(SKF525A)后碱化,乙醚萃取,三氟醋酸酐(TFA)衍生化,GC/MS全扫描定性、内标法和工作曲线法定量分析。结果:甲基苯丙胺和氯胺酮在血浆中的线性检测范围分别为0.010 0~10.0μg.mL·1和0.010 0~20.0μg.mL·1;方法回收率为95.26%~101.10%;日内及日间相对标准差均小于15%。结论:建立了衍生化后同时测定血浆中甲基苯丙胺及氯胺酮的GC/MS定性定量分析方法,该法简便、灵敏、重复性好,适用于甲基苯丙胺和氯胺酮混合滥用中毒案例的快速鉴定。
OBJECTIVE: To investigate the simultaneous GC / MS detection and analysis of methamphetamine and ketamine in rabbits plasma. Methods: The plasma samples were alkalinized with diethylstilbestrol (SKF525A), extracted with ether, derivatized with trifluoroacetic anhydride (TFA), qualitatively analyzed by GC / MS, and analyzed by internal standard method and working curve method . Results: The linear range of detection of methamphetamine and ketamine was 0.010 0 ~ 10.0 μg.mL · 1 and 0.010 0 ~ 20.0 μg.mL · 1, respectively. The recoveries were 95.26% ~ 101.10%. The intra- and inter-day Relative standard deviation are less than 15%. Conclusion: A GC / MS qualitative and quantitative method for the simultaneous determination of methamphetamine and ketamine in plasma after derivatization was established. The method is simple, sensitive and reproducible and is suitable for the rapid identification of methamphetamine and ketamine mixed abuse poisoning cases.