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研究了杀虫安经灌胃给药后对小鼠脑组织内多巴胺及氨基酸含量的影响。将60只ICR(美国癌症研究所)小鼠随机分为空白对照、杀虫安低剂量(200 mg/kg bw)及高剂量(300 mg/kg bw)3个处理组,于灌胃后出现惊厥时断头取脑,采用高效液相色谱-电化学(HPLC-ECD)检测法测定了鼠脑组织内多巴胺(DA)及其代谢产物二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量,采用液相色谱-质谱联用(LC-MS/MS)法测定了氨基酸的含量。结果发现,两个剂量处理组鼠脑组织内DA及氨基酸含量与对照相比均有显著性差异。其中:DA比对照分别升高25.25%和31.93%(P<0.05),DOPAC分别升高23.16%和37.71%(P<0.05),天门冬氨酸(Asp)分别升高13.65%和15.07%(P<0.05),谷氨酸(Glu)分别升高20.07%和25.66%(P<0.05);而低剂量组γ-氨基丁酸(GABA)含量比对照降低32.55%(P<0.05),两个剂量处理组甘氨酸(Gly)含量分别比对照降低了13.26%和17.79%(P<0.05)。表明杀虫安导致惊厥时伴有鼠脑内多巴胺和氨基酸含量的变化。
Studied the insecticide An Jing intragastric administration of dopamine and amino acids in brain tissue content. Sixty mice from ICR (American Cancer Institute) were randomly divided into three groups: blank control group (200 mg / kg bw) and high dose (300 mg / kg bw) In seizures, the brain was decapitated and dopamine (DA) and its metabolites DOPAC and HVA were assayed by HPLC-ECD. The contents of amino acids were determined by liquid chromatography-mass spectrometry (LC-MS / MS). The results showed that two doses of the brain tissue DA and amino acid content were significantly different compared with the control. DOPAC increased by 23.16% and 37.71% (P <0.05), and Asp increased by 13.65% and 15.07% (P <0.05) (P <0.05) and glutamate (Glu) increased by 20.07% and 25.66% (P <0.05), while the content of GABA in low dose group decreased by 32.55% (P <0.05) Compared with the control group, the content of Gly in each dose group decreased by 13.26% and 17.79%, respectively (P <0.05). Show insecticidal safety lead to convulsions in rats brain dopamine and amino acid content changes.