22例临床表现为非淋球菌性尿道炎的病人作尿道分泌物的支原体分离培养和血清抗体检查。结果发现支原体阳性15例(68.2％),其中解脲支原体占60％,人型支原体占27％,解脲及人型支原体均阳性占13％。15例中9例血清抗体阳性。研究证实支原体是非淋球菌性尿道炎的重要病原体。支原体的分离培养和血清抗体检查有助于早期诊断。环丙氟哌酸和氟嗪酸对支原体治疗有效。 22 cases of clinical manifestations of non-gonococcal urethritis in patients with urethral secretions Mycoplasma isolated culture and serum antibody tests. Mycoplasma positive in 15 cases (68.2%), of which Ureaplasma urealyticum accounted for 60%, Mycoplasma hominis accounted for 27%, urea and mycoplasma were positive for 13%. Nine of 15 cases were positive for serum antibody. Studies confirm that mycoplasma is an important pathogen of non-gonococcal urethritis. Mycoplasma isolation and culture and serum antibody tests help early diagnosis. Ciprofloxacin and ofloxacin are effective in mycoplasma treatment.