本文研究了用国产硅藻土为支持物进行尿中咖啡因提取分离、用二阶紫外导数光谱测定法,变异系数为3.42％,回收率为83.6％。本方法具有快速、灵敏、准确的特点。 In this paper, urinary caffeine extraction and separation using domestic diatomite as a support was studied. The coefficient of variation (CV) was 3.42% and the recovery rate was 83.6% with second-order UV derivative spectrometry. The method is fast, sensitive and accurate.