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目的 探讨胆囊癌组织雌激素受体 (ER)及孕激素受体 (PR)表达与肿瘤生物学特性的关系 ;阐明胆囊癌病人胆囊组织雌激素受体表达与血清E2 水平的关系 ;评价其在原发性胆囊癌早期诊断及临床预后中的价值。方法 采用放射免疫法 (RIA)对 2 0例对照组及 36例原发性胆囊癌组分别进行了血清雌二醇 (E2 )及孕激素 (P)水平的测定 ;采用链酶亲和素 -生物素 -辣根氧化酶 (SP)免疫组化法对 12例对照组及 36例原发性胆囊癌组胆囊组织分别进行了ER及PR的检测。结果 ER及PR在胆囊癌组织中的阳性表达率分别为 86 .1%、75 .0 % ,且与原发性胆囊癌的组织分化程度及肿瘤临床分期有关 ;胆囊癌病人血清E2 水平与胆囊组织ER和PR阳性率及半定量分级之间呈正相关。结论 胆囊癌病人血清E2 水平的异常升高及胆囊组织中ER和PR的过度表达在胆囊癌早期即出现 ,可能在胆囊癌的启动及发展过程中起着一定的作用 ,因而在原发性胆囊癌的早期诊断中具有潜在的应用价值。
Objective To investigate the relationship between the expression of estrogen receptor (ER) and progesterone receptor (PR) in gallbladder carcinoma tissue and the biological characteristics of tumors; to clarify the relationship between estrogen receptor expression in gallbladder tissue and serum E2 levels in gallbladder cancer patients; The value of early diagnosis and clinical prognosis of primary gallbladder cancer. Methods The levels of serum estradiol (E2) and progesterone (P) in 20 cases of control group and 36 cases of primary gallbladder cancer were measured by radioimmunoassay (RIA). Biotin-horseradish oxidase (SP) immunohistochemistry was used to detect ER and PR in the gallbladder tissue of 12 cases of the control group and 36 cases of primary gallbladder carcinoma. Results The positive expression rates of ER and PR in gallbladder carcinoma were 86.1% and 75.0 %, respectively, and were related to the degree of differentiation of primary gallbladder carcinoma and the clinical stage of the tumor; serum E2 levels and gallbladder in patients with gallbladder cancer There was a positive correlation between the positive rate of tissue ER and PR and semi-quantitative grading. Conclusions Abnormal elevation of serum E2 levels in gallbladder cancer patients and excessive expression of ER and PR in gallbladder tissue occur early in gallbladder cancer and may play a role in the initiation and development of gallbladder carcinoma, thus in primary gallbladder The early diagnosis of cancer has potential application value.