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目的 了解肿瘤抑制基因p5 3在肝细胞癌中的突变情况 ,探讨乙型肝炎病毒 (HBV)感染与p5 3基因突变之间的关系。方法 提取 5 0例有乙型肝炎病毒感染史肝癌患者手术样本中的DNA ,用聚合酶链式反应 (PCR)扩增 5～ 9外显子 ,作单链构象多态性 (SSCP)分析。结果 p5 3基因突变率超过 2 6 % ,突变主要分布于 5～ 8外显子 ,5、6、7、8外显子分别有 3、3、4、3例 ,另有 4个可疑突变。结论p5 3基因突变可能是肝细胞癌的病因之一 ,而乙型肝炎病毒感染在中国肝癌患者p5 3基因突变中可能起到比较重要的作用。
Objective To understand the mutation of tumor suppressor gene p53 in hepatocellular carcinoma and to investigate the relationship between hepatitis B virus (HBV) infection and p53 mutation. METHODS: DNA was extracted from surgical specimens of 50 patients with HCC who had a history of hepatitis B virus infection. Polymerase chain reaction (PCR) was used to amplify 5-9 exons for single strand conformation polymorphism (SSCP) analysis. Results The mutation rate of p53 gene was more than 26%. The mutations were mainly distributed in 5-8 exons. There were 3, 3, 4 and 3 exons 5, 6, 7, and 8 respectively, and there were 4 more suspicious mutations. Conclusion p53 gene mutation may be one of the causes of hepatocellular carcinoma, and hepatitis B virus infection may play an important role in the p53 gene mutation in Chinese liver cancer patients.