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目的探讨人肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)对HBV稳定转染的肝癌细胞株HepG2.2.15的凋亡诱导效果。方法应用MTT法了解TRAIL对HepG2.2.15细胞的诱导效果,琼脂糖凝胶电泳、流式细胞术和Caspase-3酶活性分析TRAIL诱导HepG2.2.15细胞凋亡过程。结果TRAIL(0.1μg/mL)作用于HepG2.2.15细胞株12、24、36h后,细胞抑制率分别为17.91%、41.26%、59.85%;PI单染亚二倍体含量12h后为21.8%,高于对照组的8.7%;24h后DNA电泳200bp处出现特异性条带;6h后Caspase-3酶活性为对照组的4.76倍。结论TRAIL诱导后,HBV稳定转染的肝癌细胞株HepG2.2.15发生凋亡。
Objective To investigate the apoptosis-inducing effects of human tumor necrosis factor-related apoptosis inducing ligand (TRAIL) on HepG2.2.15 hepatoma cell line stably transfected with HBV. Methods The induction effect of TRAIL on HepG2.2.15 cells was studied by MTT assay. The apoptosis of HepG2.2.15 cells was analyzed by agarose gel electrophoresis, flow cytometry and Caspase-3 activity assay. Results The inhibitory rates of TRAIL (0.1μg / mL) on HepG2.2.15 cells were 17.91%, 41.26% and 59.85% at 12, 24 and 36 h, respectively. The percentage of subunit of PI subunit was 21.8% Which was higher than that of the control group (8.7%). Specific bands appeared at 200 bp after DNA electrophoresis 24h and Caspase-3 activity was 4.76 times of the control group after 6 hours. Conclusion After the induction of TRAIL, HepG2.2.15 cells stably transfected with HBV were apoptosis.