类成骨细胞UMR106.06是一骨肉瘤细胞系,它具有许多成骨细胞的特征。作者应用激光扫描成象系统观察单个细胞证实UMR106.06细胞具有含量不同的嘌呤/核苷酸两类受体。用fluo-3/AM(6μM)标记体外培养UMR106.06细胞,彻底洗涤后,37±1℃条件下,在平皿中加入10mlα-基础培养液进行试验,检测器和激光扫描装置与Bio-Rad Osteoblast-like UMR106.06 is a osteosarcoma cell line that has many osteoblastic features. The authors applied laser scanning imaging system to observe individual cells confirmed UMR106.06 cells with different levels of purine / nucleotide two types of receptors. UMR106.06 cells were cultured in vitro with fluo-3 / AM (6μM). After thorough washing, 10 ml of α-basal medium was added to the plate under the conditions of 37 ± 1 ℃ for testing. The detector and laser scanning equipment were integrated with Bio-Rad