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对吡咯类含氮化合物的两种分离方法以及两步分离法中使用国产与进口试剂的实验结果进行了对比与评价。结果表明，分离方法不同，所得吡咯类含氮化合物总体馏分的丰度也不同，一步分离法普遍低于两步法；不同分离方法对吡咯类化合物相对分布的影响较小。国产与进口试剂性能不影响吡咯类含氮化合物的绝对丰度与相对分布的分析结果，两步分离法具备分析试剂国产化的条件。
The two separation methods of pyrrole nitrogen compounds and the experimental results of using domestic and imported reagents in the two-step separation method were compared and evaluated. The results showed that the separation methods were different, and the abundances of the overall fractions of pyrrole-like nitrogen compounds were also different. The one-step separation method was generally lower than the two-step method. The different separation methods had little effect on the relative distribution of pyrrole compounds. The properties of domestic and imported reagents do not affect the absolute abundance and relative distribution of pyrrole-containing nitrogen compounds. The two-step separation method has the advantage of analyzing the localization of reagents.