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致突变试验已广泛应用于多种环境污染物的检测。近年来,在各种致突变试验中,鱼外周血红细胞微核试验在水体污染检测中已开始应用,并具有特别的意义。由于鱼外周血红细胞体积较大,胞质丰富,核质之比为1:6,有利于胞浆中出现微核时观察。目前,以鱼为实验动物,取其外周血,观察红细胞微核,进行诱变性研究已日益引起人们关注。但是,对如何选择染毒次数和采血时间,才能获得最高的微核率,尚未见有系统的研究报道。为此,我们对鱼外周血红细胞微核率与染毒时间。染毒次数关系进行了
Mutagenicity test has been widely used in the detection of a variety of environmental pollutants. In recent years, in a variety of mutagenicity tests, the peripheral blood erythrocyte micronucleus test has begun to be used in the detection of water pollution and has special significance. Due to the larger volume of fish peripheral blood red blood cells, abundant cytoplasm, nuclear mass ratio of 1: 6, conducive to the appearance of micronuclei in the cytoplasm observed. At present, taking fish as experimental animals, taking the peripheral blood and observing erythrocyte micronucleus, mutagenicity research has drawn more and more attention. However, there is no systematic study on how to choose the maximum number of exposure times and the time of collecting blood in order to obtain the highest micronucleus rate. To this end, we fish the peripheral blood erythrocyte micronucleus rate and exposure time. The relationship between the number of injections was carried out