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维生素E(Vitamin E)作为饲料添加剂,在增强动物抗病能力、改善生产性能及提高种畜的繁殖性能等方面具有重要作用[1]。目前,国内外测定维生素E常用的有分光光度法、荧光法、气相色谱法、双波电压法、示波极谱法等。通常分光光度法的灵敏度较低,干扰比较多;荧光比色法操作比较繁琐;气相色谱法需要衍生化步骤,检测成本高。因此,确认一种准确、快速的液相色谱法测定饲料中维生素E含量对于优
As a feed additive, Vitamin E plays an important role in enhancing animal disease resistance, improving production performance and breeding performance of breeding stock. At present, the domestic and international determination of vitamin E commonly used spectrophotometry, fluorescence, gas chromatography, two-wave voltage method, oscillopolarography and so on. Usually, the sensitivity of the spectrophotometry is lower and the interference is more; the fluorescence colorimetric method is cumbersome to operate; the gas chromatography needs the derivatization step and the detection cost is high. Therefore, to confirm an accurate and rapid liquid chromatography determination of vitamin E content in feed for the excellent