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在现代医学及分子生物学研究中 ,证实、分离、鉴定差异表达基因有着极其广泛的应用。发现差异表达基因 ,有助于阐明疾病发生的分子机制、临床诊断及预后的预测 ;并是基因药物和基因治疗发展的基础。然而 ,当前克隆、鉴定差异表达基因的技术中 ,仅少数几种技术利用了人类及模式生物基因组研究业已取得的成就。其中 ,基因表达的系列分析 (serialanaly sisofgeneexpression ,SAGE)、cDNA微点阵 (cDNAmicroarrays)和综合性基因鉴定程序 (integratedprocedureforgeneidentification ,IP GI)等方法将会变得更为有利。本文将简述近年来用于寻找差异表达基因的几种主要技术 ,并对其趋势作一展望。
In the field of modern medicine and molecular biology, it has been proved that the isolation and identification of differentially expressed genes have an extremely wide range of applications. The discovery of differentially expressed genes helps to elucidate the molecular mechanisms of disease, clinical diagnosis and prognosis prediction; and is the basis for the development of gene therapy and gene therapy. However, only a few techniques currently cloned and identified differentially expressed genes have taken advantage of the achievements made in human and model organism genome research. Among them, serial analysis of gene expression (SAGE), cDNA microarrays (cDNA microarrays) and integrated gene identification procedures (integrated GI for surentification, IP GI) and other methods will become more favorable. This article will outline several major techniques used to find differentially expressed genes in recent years and outline their trends.