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目的：探讨六亚甲基二乙酰胺（ＨＭＢＡ）联合辐射对人粘液表皮样癌ＭＥＣ－１细胞超微结构的作用特点。方法：实验分４组，对照组细胞未经药物及射线处理；ＨＭＢＡ组细胞经１ｍｍｏｌ／Ｌ的ＨＭＢＡ诱导作用９６ｈ；γ－射线组细胞在乏氧状态下接受７Ｇｙ的６０Ｃｏγ－射线照射；联合应用组细胞按上述方法先经ＨＭＢＡ处理再接受辐射，用扫描电镜和透射电镜观察各组细胞的形态变化。结果：ＨＭＢＡ组细胞表面微绒毛减少，胞浆线粒体增多；γ－射线组细胞微绒毛凝聚，线粒体肿胀、空化；联合应用组细胞损伤明显，微绒毛凝聚、崩解，线粒体粘连、缺嵴、空化和紊乱。结论：ＨＭＢＡ能增强γ－射线对ＭＥＣ－１细胞超微结构的破坏作用。
Objective: To investigate the effect of HMBA combined with radiation on the ultrastructure of human mucoepidermoid carcinoma cell line MEC-1. Methods: The experiment was divided into 4 groups, the control group of cells without drug and radiation treatment; HMBA group of cells by 1mmol / L of HMBA induced 96h; γ-ray group of cells under hypoxia 7Gy 60Coγ-ray irradiation; combined application Group cells were treated with HMBA as above and then irradiated. The morphological changes of the cells were observed by scanning electron microscopy and transmission electron microscopy. Results: The microvilli and cytoplasmic mitochondria increased on the surface of HMBA group. Microvilli coalescence and mitochondria swelling and cavitation occurred in γ-ray group. The cells in combination group had obvious cell damage, aggregation and disintegration of microvilli, mitochondria adhesion, Cavitation and disorders. Conclusion: HMBA can enhance the damaging effect of γ-rays on the ultrastructure of MEC-1 cells.