目的:应用小鼠皮肤乳头状瘤生成模型,验证复方冬菊对化学致癌剂的对抗作用。方法:ICR雄性小鼠60只,随机分成模型组、复方冬菊给药组和阳性药物(增生平)对照组。小鼠背部脱毛,涂抹致癌剂DMBA/巴豆油至实验结束。12周后清点小鼠背部乳头状瘤数,以各组荷瘤动物占该组存活动物数的百分比值计算肿瘤发生率,用各组小鼠乳头瘤发生总数和该组存活小鼠数计算每鼠平均荷瘤数。比较复方冬菊给药组、阳性药物对照组和模型组肿瘤发生情况,评价药物对肿瘤生长的抑制作用。结果:实验第6周,模型组小鼠背部开始出现乳头状瘤,每鼠平均荷瘤数1.4个;复方冬菊给药组于第8周开始出现肿瘤,复方冬菊给药组和阳性药物对照组每鼠平均荷瘤数较模型组下降,分别为0.25个和0.5个,抑瘤率分别达到82.1%和64.3%。结论:复方冬菊能在一定程度上推迟肿瘤发生时间,减少小鼠皮肤乳头状瘤的发生。可见,复方冬菊对化学致癌剂有一定的对抗作用。 OBJECTIVE: To verify the antagonistic effect of compound Dongju on chemical carcinogens using mouse skin papilloma formation model. METHODS: A total of 60 ICR male mice were randomly divided into model group, compound winter chrysanthemum group and positive drug (hyperplasia) control group. The mice were depilated on the back and applied carcinogen DMBA/croton oil until the end of the experiment. After 12 weeks, the number of papillomas on the back of the mice was counted. The incidence of tumors was calculated as percentage of the number of surviving animals in each group. The total number of mouse papillomas in each group and the number of surviving mice in each group were calculated. The average number of tumors in mice. The occurrence of tumors in the Compound Winterjue group, the positive drug control group, and the model group was compared to evaluate the inhibitory effect of the drug on tumor growth. RESULTS: At the 6th week of the experiment, papillary tumors began to appear on the back of the mice in the model group. The average number of tumors per mouse was 1.4. The compound winter cyperum administration group began to develop tumors in the 8th week, and the Compound Winterjue group and positive drugs were administered. In the control group, the average number of tumors per rat was lower than that of the model group, which was 0.25 and 0.5 respectively, and the tumor inhibition rates reached 82.1% and 64.3%, respectively. Conclusion: Compound Dong Ju can delay the occurrence of tumor to a certain extent and reduce the occurrence of mouse papilloma. It can be seen that compound winter chrysanthemum has a certain antagonistic effect on chemical carcinogens.