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目的评价赶黄草的食用安全性。方法依据食品安全性毒理学评价程序和方法进行大、小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓细胞微核试验、小鼠精子畸形试验)、大鼠30 d喂养试验、大鼠致畸试验。结果大、小鼠急性经口MTD值均大于15 000 mg/kg.BW,属无毒级。3项遗传毒性试验结果未见赶黄草致突变作用。大鼠30d喂养试验结果显示,动物体重增重、进食量、食物利用率、脏体比值、血液学指标及末期血生化指标检测结果与阴性对照组比较,除雄鼠高剂量组嗜酸性细胞降低差异有统计学意义(F=2.885,P<0.05),其余各项指标差异均无统计学意义,对大鼠脏器组织均未观察到有害作用。大鼠致畸试验结果显示,赶黄草对大鼠未见母体毒性、胚胎毒性和致畸作用。结论受试赶黄草在本试验条件下未显示有急性毒性、遗传毒性、亚急性毒性或致畸作用。
Purpose To evaluate the food safety of Methods Acute oral toxicity test, genotoxicity test (Ames test, mouse bone marrow micronucleus test, mouse sperm abnormality test) and rat 30 d feeding test were conducted according to food safety toxicology evaluation procedure and method. Teratogenicity test in rats. The results of large, acute oral MTD value in mice are greater than 15 000 mg / kg.BW, is a non-toxic grade. Three genotoxicity test results showed no cataplasma mutation. The results of 30 days feeding test showed that the body weight gain, food intake, food utilization rate, dirty body ratio, hematological indexes and end-stage blood biochemical indexes test results compared with the negative control group, the eosinophils decreased The difference was statistically significant (F = 2.885, P <0.05), the rest of the indicators were no significant difference in the organs of rats were not observed in any adverse effects. Teratogenicity test results showed that rats did not catch maternal toxicity, embryotoxicity and teratogenic effects. Conclusions The test Phellodendron showed no acute toxicity, genotoxicity, subacute toxicity or teratogenicity under the experimental conditions.