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本文目的是用原代培养的神经细胞探讨知母皂甙元（ＺＭＳ）对神经细胞Ｍ受体的调节作用。主要方法是：用原代培养的神经细胞，用Ｍ受体非选择性拮抗剂３Ｈ－ＱＮＢ结合分析法测定Ｍ受体密度，并以Ｍ受体不可逆阻断剂苯甲基偶酰基胆碱氮芥（ＢＣＭ）为工具，测定Ｍ受体代谢动力学参数。主要结果：向不同培养天数（９～１５天）的神经细胞加入ＺＭＳ（０．１ｍｍｏｌＬ－１），作用４８小时后对Ｍ受体密度均有显著的上调作用（Ｐ＜０．０１）。各种浓度的ＺＭＳ（０．１～１００ｕｍｏｌＬ－１）对３Ｈ－ＱＮＢ的结合均无明显抑制作用。用（０．１ｍｍｏｌＬ－１）的ＺＭＳ显著升高Ｍ受体的合成速率（Ｐ＜０．０１），而对降解速率常数无影响（Ｐ＞０．０５）。结论：ＺＭＳ能使培养９～１５天的原代配养的大鼠神经细胞的Ｍ受体密度上调，这种作用和激动剂或拮抗剂不同，并非ＺＭＳ直接作用于Ｍ受体结合位点所引起，而是由于ＺＭＳ能促进Ｍ受体的生成。离体培养的环境相对衡定，可以排除神经体液的间接作用。
The purpose of this study was to investigate the regulatory effect of timosaponin (ZMS) on neuronal M receptors using primary cultured neurons. The main method is: using primary cultured neurons, M receptors non-selective antagonist 3H-QNB binding assay to determine M receptor density, and M receptor irreversible blocker benzoylcholine nitrogen Mustard (BCM) was used as a tool to measure the metabolic parameters of M receptors. MAIN RESULTS: ZMS (0.1 mmol L-1) was added to neural cells of different culture days (9-15 days), and the M receptor density was significantly up-regulated after 48 hours (P<0.01). Various concentrations of ZMS (0.1-100umolL-1) had no significant inhibitory effect on the binding of 3H-QNB. Using (0.1 mmol L-1) ZMS significantly increased the rate of M receptor synthesis (P<0.01), but had no effect on the degradation rate constant (P>0.05). Conclusion: ZMS can increase the density of M receptors in cultured rat neural cells cultured for 9 to 15 days. This effect is different from that of agonists or antagonists. It is not that ZMS acts directly on the M receptor binding site. Caused, but because ZMS can promote the production of M receptors. The relative culture environment is relatively constant and can exclude the indirect effects of neurohumoral fluids.