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本文就显微镜盖玻片法和常用的细胞离心制片法（Ｃｙｔｏｓｐｉｎ）对ＢＡＬＦ细胞分数的测定进行比较，同时探讨了ＢＡＬＦ的不同处理方法对细胞总、分数的影响。结果表明：Ｃｙｔｏｓｐｉｎ制片淋巴细胞（％）明显低于盖玻片法，但两种制片方法测得的中性粒细胞和嗜酸粒细胞均相近；离心和洗涤明显丢失细胞总数（Ｐ＜０．０１），但不限于单一细胞型。本研究提示：用盖玻片法测定ＢＡＬＦ细胞分数和在非离心、洗涤的ＢＡＬＦ中测定细胞总数，能准确地反映ＢＡＬＦ中细胞的数目和类型。
In this paper, we compared the determination of BALF cell fraction by microscope coverslip method and Cytospin, and discussed the effect of different treatment methods on the total cell and fraction of BALF. The results showed that the percentage of lymphocytes (%) of Cytospin preparation was significantly lower than that of cover glass, but the neutrophil and eosinophil count were similar in both methods. The total number of cells lost obviously in centrifugation and washing (P < 0.01), but not limited to a single cell type. This study suggests that determining the number of BALF cells using the coverslip method and the total number of cells in a non-centrifuged, washed BALF accurately reflect the number and type of cells in the BALF.