论文部分内容阅读
目的研究Nel样分子I型(nel-like typeⅠmolecular,NELL1)对大鼠脂肪干细胞(adipose-derived stem cells,ADSCs)增殖和分化的影响。方法分离培养大鼠ADSCs并进行传代;在成骨诱导条件下,以脂质体为载体,分别搭载NELL1质粒和增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)质粒作为实验组和对照组,转染第三代ADSCs;通过MTT、RT-q PCR、碱性磷酸酶(alkaline phosphatase,ALP)活性以及茜素红染色方法检测细胞增殖和分化能力;采用单因素方差分析进行统计学运算。结果实验组与对照组对比,细胞数量差异无统计学意义(P>0.05),ALP活性及钙结节形成量实验组明显高于对照组。第3d和第7d时,实验组成骨相关基因Runx2、ColⅠ、ALP表达增加(P<0.01),14d时ColⅠm RNA表达增加(P<0.05)。结论 NELL1对ADSCs增殖无明显影响,促进其向成骨细胞分化。
Objective To investigate the effect of Nel1 (NELL1) on proliferation and differentiation of rat adipose-derived stem cells (ADSCs). Methods ADSCs were isolated and cultured in vitro. Under osteogenic conditions, NELL1 plasmid and enhanced green fluorescent protein (EGFP) plasmids were respectively used as the experimental group and the control group, The third generation of ADSCs were transfected. The proliferation and differentiation of cells were detected by MTT, RT-q PCR, alkaline phosphatase (ALP) activity and alizarin red staining. Statistical analysis was performed by one-way ANOVA. Results There was no significant difference in the number of cells between the experimental group and the control group (P> 0.05). ALP activity and calcium formation in the experimental group were significantly higher than those in the control group. On the 3rd and 7th day, the expressions of osteocalcin-related genes Runx2, ColⅠ and ALP increased (P <0.01), and the expression of ColⅠmRNA increased on the 14th day (P <0.05). Conclusion NELL1 has no obvious effect on the proliferation of ADSCs and promotes its differentiation into osteoblasts.