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应用对皮肤表面涂敷的方法免疫，而不用针头穿刺身体，将会大大增加疫苗接种的容易程度。经口腔和鼻腔施用疫苗时，细菌产物霍乱毒素（ＣＴ）常被用以加强免疫应答。我们发现，当把ＣＴ施用到皮肢表面后，ＣＴ刺激了机体对疫苗成份如白喉类毒素或破伤风类毒素的免疫应答。因此
Application of the method of coating the skin surface immunity, without needle puncture the body, will greatly increase the ease of vaccination. The bacterial product cholera toxin (CT) is often used to boost the immune response when the vaccine is administered orally and nasally. We found that CT stimulates the body’s immune response to vaccine components such as diphtheria toxoid or tetanus toxoid when CT is applied to the surface of the limb. therefore