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[目的]了解四川5.12地震后板房学校中小学学生流行性脑膜炎带菌(Neissria Meningitides Nm)状况,评价震后初期防疫效果,为预测疫情与制定防治措施提供依据。[方法]按分层整群抽样法,采集绵阳市某县中、小学学生咽拭子样本共614份,常规分离培养鉴定Nm、荧光定量PCR测定Nm核酸、血清学分型。[结果]37人分离到Nm,带菌率为6.03%;荧光定量PCR法测定Nm核酸38份咽拭子阳性,阳性率为6.19%;B群35株(92.1%),W135群2株(5.3%),一株未分出群(2.7%)。性别带菌率差异无统计学意义,不同年龄组之间差异有统计学意义。15～20岁组Nm带菌率最高。[结论]震后灾区板房学校中小学学生流脑带菌主要以B群为主。
[Objective] To understand the status of epidemic meningitis (Neissria Meningitides Nm) in primary and secondary schools in boarding schools in Sichuan after the 5.12 earthquake and to evaluate the effect of early epidemic prevention after earthquake to provide basis for forecasting epidemic situation and making prevention and control measures. [Method] According to the stratified cluster sampling method, a total of 614 throat swab samples from primary and secondary school students in a county of Mianyang were collected and routinely isolated and identified for Nm. Nm nucleic acid and serological typing were determined by fluorescence quantitative PCR. [Results] 37 of them were isolated from Nm and the rate of infection was 6.03%. The positive rate of phagocytosis of 38 pharyngeal swabs from Nm nucleic acid by fluorescence quantitative PCR was 6.19%, 35 (92.1%) in group B and 2 (5.3) %), An undistributed group (2.7%). There was no significant difference in gender-carrying rate between different age groups. The incidence of Nm in the 15-20 age group was the highest. [Conclusion] The prevalence of meningococcal carriage of primary and secondary school students in Banfang schools in the disaster-stricken area was mainly B group.