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目的:对以金龟商标注册的黄芪注射液中主要活性成分——黄芪皂甙进行定量研究。方法:以黄芪甲甙为对照品,以5％香草醛冰醋酸溶液和高氯酸为显色剂,采用比色法测定黄芪注射液中黄芪总皂甙的量。结果:在20～100μg/ml的范围内,浓度和吸收度呈良好的线性关系,r=0.9999,平均加样回收率为105.6％(n=5),RSD=1.74％。结论:本法显色灵敏度高,重现性好,适用于生产过程中的中间质量监测和成品的定量。
OBJECTIVE: To quantitatively study the main active ingredient of Astragalus membranaceus in Astragalus injection registered with the trade name of Turtle Tortoise. Methods: Astragaloside was used as the reference substance, 5% vanillin glacial acetic acid solution and perchloric acid were used as color reagents, and the amount of total saponins of Astragalus membranaceus in Astragalus membranaceus injection was determined by colorimetry. RESULTS: In the range of 20-100 μg/ml, the concentration and the absorption showed a good linear relationship, r=0.9999, the average recovery was 105.6% (n=5), and the RSD was 1.74%. Conclusion: This method has high color sensitivity and good reproducibility, and is suitable for intermediate quality monitoring and finished product quantification in the production process.