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目的幽门螺杆菌（Ｈｐ）在体外很易发生球形变，目前一般认为是不能培养的退化状态．而对Ｈｐ在环境中生存方式和如何感染大量人群仍不清楚，为此本研究对Ｈｐ的形态变化尤其是球形变的返祖进行了研究．方法本研究通过对１８株Ｈｐ在不同条件下的培养，观察其生存及形态变化，并在加有鸡蛋和小牛血清的空弯培养基上及加有血清有布氏肉汤培养基中是进行培养和返祖，观察其变异规律．结果Ｈｐ可在室温液体中繁殖，１８株中观察到３株球形体Ｈｐ在室温下的返祖，除常见球形变外的Ｈｐ杆状弯曲、长杆状、短杆状、长丝体、巨球体、丝球体、原生质体及细小“Ｓ”和弧形变异．并根据Ｈｐ形态变化的顺序及表型，初步发现Ｈｐ由典型“Ｓ”或多螺旋形态变为杆状弯曲、长杆状、短杆状、然后变为球形、长丝体、巨球体、丝球体、原生质体的规律．部分变异体在一定条件下可返祖．结论Ｈｐ易发生多形变化，有一定规律．可在室温下生长繁殖，球形体可在Ｈｐ鸡蛋培养基上或室温液体培养基中返祖．提示Ｈｐ的球形变在其感染和治疗后的复发中起重要作用
Purpose Helicobacter pylori (Hp) in vitro is prone to spherical degeneration, is currently considered to be unable to cultivate the degenerative state. However, it is still unclear how Hp will survive in the environment and how to infect a large number of people. To this end, morphological changes of Hp, especially spherical anomie were studied. Methods In this study, 18 Hp strains were cultured under different conditions to observe their survival and morphological changes, and in empty medium supplemented with egg and calf serum on the curved medium and added with serum Brinell broth medium To cultivate and return to ancestors, observe the variation of law. Results Hp could be propagated in liquid at room temperature. In 18 of 18 strains, the anomalous Hp of three spheroids were observed at room temperature. In addition to the common spherical deformation, Hp rod bending, long rod, short rod, filament, giant Spheroids, spheroids, protoplasts, and small “S” and arc variants. According to the order and phenotype of Hp morphological changes, it was found that Hp changed from typical “S” or multi-helical shape to rod-shaped bend, long rod shape and short rod shape, and then turned into spherical shape, filament body, giant sphere and silk Spheres, protoplasts of the law. Some variants can return to ancestors under certain conditions. Conclusion Hp prone to polymorphism, there are certain rules. It can grow and reproduce at room temperature. Spheroids can be returned on Hp egg medium or in room temperature liquid medium. Suggesting that Hp globular transformation plays an important role in the relapse after infection and treatment