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目的探讨不同营养模式对宫内发育迟缓幼鼠胰岛素样生长因子及受体表达的影响。方法用母鼠全程饥饿法建立宫内发育迟缓(IUGR)动物模型,IUGR新生雌鼠随机分为4组,新生幼鼠母乳喂养,各组分别给予母鼠下述相应饮食饲料喂养3周至断乳:①IUGR对照组给予常规饮食;②IUGR高糖饮食组(A组);③IUGR高蛋白饮食组(B组);④IUGR高脂饮食组(C组)。各组幼鼠在3周哺乳期间母鼠分别摄取上述饲料。各组幼鼠断乳后同样分别摄取上述饲料。另以正常母鼠所生正常新生雌鼠为正常对照组(C/N组)。生后母乳喂养至3周断乳,母鼠及断乳后幼鼠均给予常规饮食喂养。各组幼鼠分别取肝、肺进行免疫组织化学染色检测和胰腺组织进行苏木精-伊红染色检测。结果幼鼠多肽糖金属蛋白酶12(ADAM12)的测定结果各个月之间均无统计学意义(P>0.05)。幼鼠孕期相关血浆蛋白酶A(PAPP-A)的测定结果在各组幼鼠的第1个月有统计学意义(P<0.05),但在第2、3、4个月无统计学意义(P>0.05)。幼鼠Toll样受体(TLR-4)的测定结果各个月之间均无统计学意义(P>0.05)。幼鼠胰腺组织结果在幼鼠的第1个月有统计学意义(P<0.05),但在第2、3、4个月无统计学意义(P>0.05)。结论 ADAM12和PAPP-A的表达量或许与幼鼠的追赶生长密切相关;尽管TLR-4的检测结果在统计学上无明显意义,但高糖饮食组和高脂饮食组胰腺发生炎症的可能性较大。
Objective To investigate the effects of different nutrition modes on the expression of insulin-like growth factor and its receptors in pups with intrauterine growth retardation. Methods The intrauterine growth retardation (IUGR) animal models were established by full starvation of the female rats. The newborn female rats were randomly divided into 4 groups. The newborn rats were fed with breast milk. The rats were fed with the following diets for 3 weeks respectively. ①UGR high-sugar diet group (group A); ③UGR high protein diet group (group B); ④IUGR high-fat diet group (group C). Each group of young rats during the three weeks of lactation were taken in the above-mentioned feed. Each group of young rats were weaned after the same intake of the above feed. Another normal female born normal newborn female rats as a normal control group (C / N group). Breastfeeding after birth to three weeks after weaning, maternal and post-weaning young rats were given conventional diet. Each group of young rats were taken liver and lung for immunohistochemical staining and pancreatic tissue for hematoxylin-eosin staining. Results There was no significant difference in the results of ADAM12 assay between months (P> 0.05). The result of PAPP-A in pregnancy was statistically significant at the first month (P <0.05), but not in the second, third and fourth month P> 0.05). Toll-like receptor (TLR-4) in young rats results were not statistically significant between months (P> 0.05). The result of pancreas in young rats was statistically significant at the first month (P <0.05), but not at the second, third and fourth month (P> 0.05). Conclusions The expression of ADAM12 and PAPP-A may be closely related to the catch-up growth in young rats. Although the results of TLR-4 are not statistically significant, the possibility of inflammation in the pancreas of high-glucose diet group and high-fat diet group Larger.