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以“青芋2号”茎段为试验材料,考察了不同培养基对菊芋愈伤组织诱导、丛生芽诱导及生根培养的影响,并利用已优化的SSR-PCR反应体系对不同继代次数的菊芋试管苗进行遗传稳定性分析。结果表明:适宜菊芋离体增殖的培养基植物生长激素调节物质配比为MS+1mg/L6-BA+0.5mg/L NAA,适合菊芋生根培养基最佳配比为1/2MS+0.1mg/L IBA。从继代8次的组培苗中分析其遗传的变化,在检测出的50个条带中,个体之间未发生变异,生物学性状上亦未发现明显差异。试验表明在多次继代培养过程后,菊芋的遗传物质未发生明显变化,为今后菊芋快繁及离体保存等提供了技术参考。
The effects of different medium on the callus induction, shoot induction and rooting of Jerusalem artichoke were investigated using stem segments of “Amomum No. 2” as experimental materials. The effects of different medium on callus induction, The number of Jerusalem artichoke tube plantlets for genetic stability analysis. The results showed that the optimum ratio of plant growth hormone regulators (MS + 1 mg / L 6-BA + 0.5 mg / L NAA) L IBA. Genetic changes were analyzed from the tissue culture seedlings of 8 generations after subculture. Among the 50 bands detected, no variation occurred among individuals, and no significant difference was found in biological traits. Experiments show that after repeated subcultures, the genetic material of Jerusalem artichoke did not change significantly, which provided a technical reference for rapid propagation and isolation of Jerusalem artichoke in the future.