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1997年 6月在山东省日照市岚山头附近海域收集日本鬼 ,采用放射免疫学方法检测了注射毒腺粗提物大鼠局部肌肉组织、血浆、脊髓、延髓、中脑、下丘脑及免疫器官内P物质和生长抑素含量变化 ;用MTT法检测了毒腺粗提物在整体和离体条件下对免疫细胞活性的影响 ;用生化方法检测了毒腺组织中透明质酸酶活性。结果表明 ,与对照组大鼠相比 ,实验组大鼠局部肌肉及延髓、中脑、胸腺和脾脏中P物质含量增加 ,而下丘脑中P物质含量下降。生长抑素在中脑、延髓、脊髓、胸腺中显著增高。毒腺粗提物对胸腺细胞和脾细胞的活性具有显著的抑制作用。提示日本鬼蜇伤造成局部症状及其伴随的恶心、呕吐、心慌、胸闷等全身症状和继发感染可能与P物质和生长抑素含量变化有关。
In June 1997, Ginkgo biloba was collected from the sea near Arashiyama in Rizhao, Shandong Province. Radioimmunology was used to detect the local muscle tissue, plasma, spinal cord, medulla oblongata, midbrain, hypothalamus and immune organs The contents of substance P and somatostatin in the gonadotrophs. The effects of crude extract of venom gland on the activity of immune cells were detected by MTT assay. The activity of hyaluronidase in the glandular gland was detected by biochemical methods. The results showed that compared with the control group, the content of substance P in local muscle, medulla oblongata, midbrain, thymus and spleen of rats in experimental group increased while the content of substance P in hypothalamus decreased. Somatostatin in the midbrain, medulla, spinal cord, thymus was significantly higher. Venom gland extract on thymus cells and spleen cell activity was significantly inhibited. Prompted the Japanese ghost sting caused by local symptoms and its accompanying nausea, vomiting, palpitation, chest tightness and other systemic symptoms and secondary infection may be related to substance P and somatostatin content changes.