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已知缺氧预处理不仅对心肌细胞，而且对血管床亦有保护作用；但对血管壁细胞是否有直接保护作用，目前尚不清楚。本工作在培养的家兔血管平滑肌细胞（ＶＳＭＣ）缺氧复氧（Ａ／Ｒ）损伤模型上观察缺氧预处理（ａｎｏｘｉｃｐｒｅｃｏｎｄｉｔｉｏｎｉｎｇ，ＡＰＣ）的影响。发现ＡＰＣ能提高Ａ／Ｒ后ＶＳＭＣ存活率，减轻细胞脂质过氧化损伤和钙超载，使用蛋白激酶Ｃ（ＰＫＣ）激动剂ＰＭＡ能模拟，而抑制剂Ｈ７或ｐｏｌｙｍｙｘｉｎＢ能完全消除ＡＰＣ的上述保护作用。提示ＡＰＣ对ＶＳＭＣ的Ａ／Ｒ损伤具有保护作用，其机理可能与ＰＫＣ激活有关
It is known that hypoxic preconditioning not only protects cardiomyocytes but also vascular beds, but it is still unclear whether there is a direct protective effect on vascular wall cells. In this study, we observed the effects of anoxic preconditioning (APC) on cultured rabbit vascular smooth muscle cells (VSMCs) under hypoxia-reoxygenation (A / R) injury model. It was found that APC can improve VSMC survival after A / R, reduce cell lipid peroxidation and calcium overload, and can be mimicked by protein kinase C (PKC) agonist PMA, while inhibitor H7 or polymyxin B can completely eliminate the above protective effect of APC . Tip APC VSMC A / R injury has a protective effect, the mechanism may be related to the activation of PKC