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目的:观察髓过氧化物酶抑制剂氨基苯甲酰肼对中性粒细胞与内皮细胞黏附的影响。方法:应用Percoll密度梯度离心法分离人中性粒细胞;黏附实验观察髓过氧化物酶抑制剂氨基苯甲酰肼和抗CD11b抗体对中性粒细胞与内皮细胞黏附的影响。结果:急性心肌梗死患者中性粒细胞与内皮细胞黏附率为(47.63±10.16)%,高于健康对照组的(8.82±2.63)%(P<0.01);氨基苯甲酰肼浓度在0、5、10、20μmol/L时二者的黏附率分别为(47.63±10.16)%、(43.94±9.91)%、(35.13±6.95)%和(26.01±7.24)%,而仅10、20μmol/L组与0μmol/L组比较差异有统计学意义(P<0.05,P<0.01);抗CD11b抗体组的黏附率为(19.63±6.38)%明显低于0μmol/L组(P<0.01)。结论:髓过氧化物酶抑制剂氨基苯甲酰肼对急性心肌梗死患者中性粒细胞与内皮细胞的黏附具有抑制作用,且呈剂量依赖性,可能与氨基苯甲酰肼影响中性粒细胞表面CD11b表达有关。
Objective: To observe the effect of myeloperoxidase inhibitor aminobenzohydrazide on the adhesion of neutrophils and endothelial cells. METHODS: Percoll density gradient centrifugation was used to separate human neutrophils. Adhesion was used to observe the effect of myeloperoxidase inhibitor aminobenzohydrazide and anti-CD11b antibody on the adhesion of neutrophils and endothelial cells. Results: The adhesion rate of neutrophils and endothelial cells in acute myocardial infarction patients was (47.63 ± 10.16)%, which was higher than that in healthy controls (8.82 ± 2.63)% (P <0.01) The adhesive rates of the two groups were (47.63 ± 10.16)%, (43.94 ± 9.91)%, (35.13 ± 6.95)% and (26.01 ± 7.24)%, respectively, while those of 10, 20μmol / (P <0.05, P <0.01). The adhesion rate of anti-CD11b antibody group (19.63 ± 6.38)% was significantly lower than that of 0μmol / L group (P <0.01). Conclusion: The myeloperoxidase inhibitor aminobenzohydrazide can inhibit the adhesion of neutrophils to endothelial cells in acute myocardial infarction patients in a dose-dependent manner and may be related to the effect of aminobenzohydrazide on the neutrophil Surface CD11b expression.