The hyperspectral reflectances of the canopy, the first and the third unfolding leaves from the top and the panicles of two rice varieties (Xiushui 110 and Xieyou 9308) were measured by a ASD FieldSpec Pro FR?in field and indoor environments under three nitrogen levels at different developmental stages. The concentrations of chlorophyll and carotenoid in leaves and panicle corresponding to the spectra were determined by biochemical method. The spectral differences were significant for rice under different nitrogen levels, and the concentrations of chlorophyll and carotenoid in leaves increased along with increasing applied nitrogen. There were more pronounced differences for the pigment concentrations in rice leaves with different nitrogen levels. The spectra] reflectance of canopy was gradually getting smaller in the visible region and bigger in the near infrared region as the growth stages followed before heading, but with reverse change after heading. There existed 'blue shift' phenomena for the position of red edge in the spectra of canopy, leaves and panicle after heading. The concentrations of chlorophyll and carotenoid in leaves presented S-shape change. The concentrations of chlorophyll and carotenoid in canopies, leaves and panicles were highly significantly correlated to the hyperspectral vegetation indices (Vis) R990/R553, R1200/R553, R750/R553,R553/R670/R800/R553, PSSRa, PSNDa and the red edge position λred, indicating that these Vls could be used to estimate the concentrations of chlorophyll and carotenoid in canopies, leaves and panicles of rice. The hyperspectral reflectances of the canopy, the first and the third unfolding leaves from the top and the panicles of two rice varieties (Xiushui 110 and Xieyou 9308) were measured by a ASD FieldSpec Pro FR? In field and indoor environments under three nitrogen levels at The concentrations of chlorophyll and carotenoid in leaves and panicle corresponding to the spectra were determined by biochemical method. The spectral differences were significant for rice under different nitrogen levels, and the concentrations of chlorophyll and carotenoid in leaves increased along with increasing applied There are more pronounced differences for the pigment concentrations in rice leaves with different nitrogen levels. The spectra] reflectance of canopy was gradually getting smaller smaller in the visible region and bigger in the near infrared region as the growth stages followed before heading, but with reverse changed after heading. There existed 'blue shift' phen The concentrations of chlorophyll and carotenoid in leaves presented S-shape change. The concentrations of chlorophyll and carotenoid in canopies, leaves and panicles were highly predominant to the hyperspectral vegetation indices (Vis) R990 / R553, R1200 / R553, R750 / R553, R553 / R670 / R800 / R553, PSSRa, PSNDa and the red edge position λ red, indicating these Vls could be used to estimate the concentrations of chlorophyll and carotenoid in canopies, leaves and panicles of rice.