论文部分内容阅读
本文报导恶性疟原虫感染的松鼠猴红细胞表面存在抗原决定簇。实验用的原虫有两株:乌干达I/PLF-3和泰国柬埔寨-I,后者用夜猴及去脾的松鼠猴保种。以约10(?)原虫感染的红细胞静脉接种正常或去脾松鼠猴获得感染,感染血于液氮中保存。用于检查表面抗原的方法见Da-vid等。取1～15%虫血症的样品100ul,在含10%免疫血清的RPMI-16401ml中室温共育30分钟,用pH7.2的PBS洗2次,尔后加2ml PBS制成悬液。通过A蛋白琼脂糖凝胶珠柱,再用6ml PBS洗脱。流出的液体为“通过样本”。用吸管将吸附于小珠上的感染细胞在1ml PBS中反复冲洗,使之脱落,得到的液体为“栏留样本”。
This article reports the presence of antigenic determinants on the surface of erythrocytes infected with P. falciparum. Two protozoa were used for the experiment: Ugandan I / PLF-3 and Thailand-Cambodia-I, which were bred with a night monkey and a spleen-ridden squirrel monkey. Inoculated with about 10 (?) Protozoal erythrocyte venous normal or spleens squirrel infected, the infected blood was stored in liquid nitrogen. For methods of checking for surface antigens, see Da-vid et al. Take 100 ul of 1-15% parasitemia samples and incubate them in RPMI-16401 ml containing 10% immune serum for 30 minutes at room temperature, wash twice with PBS at pH 7.2 and then add 2 ml PBS to make a suspension. Pass Protein A Sepharose beads and elute with 6 ml PBS. Outflow of liquid through the “sample.” The infected cells adsorbed onto the beads were repeatedly washed in 1 ml PBS with a pipette and allowed to peel off. The resulting liquid was a “column-stay sample.”