论文部分内容阅读
目的研究静磁场暴露对大鼠血液流变学以及重要器官形态结构的影响。方法雄性Wistar大鼠80只,随机分为对照组、低剂量组(200 mT)、中剂量组(400 mT)和高剂量组(600 mT)。采用中科院电工所研制的超导磁体系统,将大鼠进行全身暴露,3 h/d,连续14 d。于暴露后1 d、7 d、14 d、28 d、3 m,检测大鼠血流动力学改变,取脑、心、肺、肝、脾、肾、胸腺、肾上腺、小肠、胰腺,常规病理制片,行光镜和电镜观察。结果 (1)磁场暴露后早期,中、低剂量组大鼠全血粘度升高;暴露后中晚期,高剂量组大鼠全血粘度降低。(2)磁场暴露后,大鼠脑、心、肺、脾、肾均见毛细血管充血或间质出血,并见实质细胞不同程度变性、凋亡和坏死。(3)上述病变均于暴露后1~14 d呈加重趋势,28 d减轻,3 m未恢复,且以高、中剂量组较为明显。结论 200 mT、400 mT及600 mT中等强度静磁场暴露均导致大鼠血液流变学异常,脑、心、肺、脾、肾多器官血液循环障碍及实质细胞不同程度变性、凋亡和坏死,且与暴露剂量密切相关。
Objective To study the effects of static magnetic field exposure on hemorheology and morphological changes of vital organs in rats. Methods Eighty male Wistar rats were randomly divided into control group, low dose group (200 mT), middle dose group (400 mT) and high dose group (600 mT). Using the superconducting magnet system developed by Chinese Academy of Sciences Institute of Electrical Engineering, rats were exposed for 3 h / d for 14 days continuously. After 1, 7, 14, 28 and 3 m of exposure, hemodynamics of the rats were measured and the brain, heart, lung, liver, spleen, kidney, thymus, adrenal gland, small intestine, pancreas and routine pathology Production, line light microscope and electron microscopy. Results (1) The viscosity of whole blood of rats in the early, medium and low dose groups after magnetic field exposure increased. The whole blood viscosity of the rats in the late, medium and high dose groups decreased. (2) After exposure to magnetic field, capillary congestion or interstitial hemorrhage was seen in the brain, heart, lung, spleen and kidney of rats, and degeneration, apoptosis and necrosis of parenchymal cells were observed. (3) The above lesions all showed a trend of aggravating from 1 to 14 days after exposure, relieved at 28 days and not recovered at 3 years, especially in high and medium dose groups. Conclusion The moderate static magnetic field exposures at 200 mT, 400 mT and 600 mT all lead to abnormal hemorheology, blood circulation disorders in brain, heart, lung, spleen and kidney, varying degree of parenchymal cell degeneration, apoptosis and necrosis, And is closely related to the dose of exposure.