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目的 探讨疏血通对沙土鼠脑缺血再灌注损伤的保护作用。方法 4 8只成年雄性沙土鼠随机分成假手术组、缺血再灌注组和治疗组 ,阻断双侧颈总动脉 7分钟后血液复流 ,制成沙土鼠脑缺血再灌注模型。每组随机取 8只沙土鼠于再灌注后 2 4小时处死 ,分离出海马区及额顶部脑组织 ,作脑组织匀浆。测定脑组织匀浆中的NO、NOS含量 ,同时对再灌注后 12小时、3天、7天海马区的病理学改变进行观察。然后比较假手术组、缺血再灌注组和治疗组上述指标的变化。结果 缺血再灌注后 12小时治疗组较缺血再灌注组海马CA1区神经元变性、坏死程度明显减轻 ,随再灌注时间的推移两组变性、坏死及形态不规则的神经元均逐渐增多 ;超微结构显示治疗组细胞器、细胞核及细胞内膜系统的损伤性改变较缺血再灌注组明显减轻。治疗组NO、NOS含量则明显低于缺血再灌注组 (P <0 .0 5 ) ,而缺血再灌注组NO、NOS含量明显高于假手术组 (P <0 .0 5 ) ;治疗组与假手术组NO、NOS含量差别无显著性 (P >0 .0 5 )。结论 疏血通可能通过抑制NO合成来对脑缺血再灌注损伤发挥保护作用。
Objective To investigate the protective effect of Shuxuetong against cerebral ischemia-reperfusion injury in gerbils. Methods 48 adult male gerbils were randomly divided into sham-operation group, ischemia-reperfusion group and treatment group. The bilateral common carotid arteries were blocked for 7 minutes and then the blood was re-flowed to make a model of gerbil cerebral ischemia-reperfusion. In each group, 8 gerbils were sacrificed at 24 hours after reperfusion. Hippocampus and frontal brain tissue were isolated and homogenized. The contents of NO and NOS in brain homogenate were measured. The pathological changes in hippocampus at 12, 3 and 7 days after reperfusion were observed. Then compare sham operation group, ischemia-reperfusion group and treatment group above index changes. Results The degeneration and necrosis of neurons in CA1 area of hippocampus in ischemia-reperfusion group were significantly reduced 12 hours after ischemia-reperfusion, the neurons in degeneration, necrosis and irregular morphology gradually increased with the reperfusion time; The ultrastructure showed that the injury changes of organelles, nuclei and endometrial system in treatment group were significantly reduced than those in ischemia-reperfusion group. The levels of NO and NOS in the treatment group were significantly lower than those in the ischemia-reperfusion group (P <0.05), while the contents of NO and NOS in the ischemia-reperfusion group were significantly higher than those in the sham operation group (P <0.05) There was no significant difference in NO and NOS between group and sham group (P> 0.05). Conclusion Shuxuetong may play a protective role in cerebral ischemia-reperfusion injury by inhibiting NO synthesis.