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目的研究不同频率和时间的振动运动对大鼠骨骼肌糖代谢的影响,并探讨适宜的振动频率和振动时间。方法 42只SD雄性大鼠,根据对其施加的不同振动频率(15、25、35 Hz)和持续时间(5min和15 min)随机分成7组:安静对照组(CC)、低频率短时间振动训练组(LS)、低频率长时间振动训练组(LL)、中频率短时间振动训练组(MS)、中频率长时间振动训练组(ML)、高频率短时间振动训练组(HS)和高频率长时间振动训练组(HL)。每组进行相应频率和时间的振动运动,每周训练6 d,持续8周。训练结束后取材,检测空腹血糖、肌糖元、肌酸激酶和肌纤维横截面积;免疫组化、Western blot检测腺苷酸活化蛋白激酶(AMPK)和葡萄糖转运蛋白4(GLUT4)的表达。结果 (1)与CC组相比,ML组血糖显著降低(P<0.05),肌纤维横截面积显著减小(P<0.05),腓肠肌糖元、肌酸激酶增加幅度最大(P<0.05)。(2)与CC组相比,各实验组AMPK-GLUT4通路蛋白的表达显著高于CC组(P<0.05),其中ML组变化最明显,显著高于其它实验组(P<0.05)。结论 (1)振动运动能够促进骨骼肌对葡萄糖的摄取利用,改善糖代谢。(2)中频率长时间的振动运动(25 Hz,15 min)最有利于促进大鼠骨骼肌细胞的糖代谢。
Objective To study the effect of vibration at different frequencies and times on the glucose metabolism in skeletal muscle of rats and to explore the suitable vibration frequency and vibration time. Methods Forty-two SD male rats were randomly divided into 7 groups according to different vibration frequency (15,25,35 Hz) and duration (5 min and 15 min): quiet control group (CC), low frequency short time vibration (LS), low frequency long time vibration training group (LL), medium frequency short time vibration training group (MS), medium frequency long time vibration training group (ML), high frequency short time vibration training group (HS) and High frequency long time vibration training group (HL). Each group of the corresponding frequency and time of vibration exercise, training 6 d per week for 8 weeks. After training, the fasting blood glucose, muscle glycogen, creatine kinase and muscle fiber cross-sectional area were measured. The expressions of AMPK and GLUT4 were detected by immunohistochemistry and Western blot. Results (1) Compared with CC group, the blood glucose in ML group was significantly decreased (P <0.05), the muscle fiber cross-sectional area was significantly reduced (P <0.05), and the gastrocnemius muscle glycogen and creatine kinase increased the most (P <0.05). (2) Compared with CC group, the expression of AMPK-GLUT4 pathway protein in each experimental group was significantly higher than that in CC group (P <0.05). ML group had the most obvious change, which was significantly higher than other experimental groups (P <0.05). Conclusion (1) Vibration can promote skeletal muscle glucose uptake and utilization, improve glucose metabolism. (2) The long-time medium frequency vibration (25 Hz, 15 min) is the most conducive to the promotion of glucose metabolism in rat skeletal muscle cells.