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目的探讨大鼠癫痫持续状态(SE)后海马结构神经型一氧化氮合酶(nNOS)、诱导型一氧化氮合酶(iNOS)及活化Caspase-3蛋白表达的动态变化及其相互关系。方法采用锂-匹罗卡品腹腔注射建立大鼠SE模型,用免疫组织化学及免疫印迹法在2h、6h、3d及7d不同时相点检测大鼠海马nNOS、iNOS及活化Caspase-3蛋白的表达。结果大鼠海马nNOS的蛋白表达在SE后2h开始迅速增高,在6h时达到高峰,之后逐渐降低,但在3、7d时仍显著高于对照组(P均<0.01)。iNOS蛋白的表达在SE后6h时开始持续增高(P<0.01),并在7d时达到高峰(P<0.01)。活化Caspase-3蛋白的表达在3d开始增高(P<0.01),7d时达到高峰(P<0.01)。结论大鼠在SE后海马的nNOS、iNOS及活化Caspase-3的表达均有不同程度的增高,提示一氧化氮合酶的反应产物一氧化氮可能与癫痫发作后海马结构内的神经元受损有关,而神经元受损与凋亡之间的关系亦密不可分。
Objective To investigate the dynamic changes of hippocampal formation of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and activated Caspase-3 protein in rats with status epilepticus (SE). Methods The SE model was established by intraperitoneal injection of lithium-pilocarpine. The expressions of nNOS, iNOS and activated Caspase-3 protein in hippocampus were detected by immunohistochemistry and Western blotting at different time points of 2h, 6h, 3d and 7d expression. Results The protein expression of nNOS in hippocampus of rats rapidly increased at 2h after SE and reached a peak at 6h, then decreased gradually, but was still significantly higher than that of control at 3 and 7 days (all P <0.01). The expression of iNOS protein began to increase at 6h after SE (P <0.01), and peaked at 7d (P <0.01). The expression of activated Caspase-3 protein increased at 3d (P <0.01), reached the peak at 7d (P <0.01). Conclusions The expression of nNOS, iNOS and activated Caspase-3 in hippocampus of rats increased to some extent after SE, suggesting that the nitric oxide synthase reaction product may be impaired in the hippocampal neurons after epileptic seizure Related, and the relationship between neuronal damage and apoptosis are also inseparable.