试验方法首先选用合适的培养基——1.5%麦芽汁琼脂培养基,但有些真菌不适合这种培养基,则用马铃薯葡萄糖琼脂培养基代替然后把试验所需的菌种接种到皮氏培养皿上作平面培养。试验用的皮氏培养皿直径一般为60～90毫米,接种时菌种应放在培养皿中心位置,培养直到菌落直径达3厘米以上,取一根经消毒过大约14厘米长的细玻璃管,用钝的一端切下一块直径为6毫米的菌种块,用锐端把切下的菌种块小心地移到2毫升的小瓶 The test method first selected a suitable medium - 1.5% wort agar medium, but some fungi are not suitable for this medium, then potato dextrose agar medium instead of the required test strains were inoculated Petri dish For plane training. Test Petri dish diameter is generally 60 to 90 mm, inoculation species should be placed in the center of the Petri dish, cultured until the colony diameter of 3 cm or more, take a sterilized about 14 cm long thin glass tube , Cut off a piece of bacteria 6 mm in diameter at the blunt end and carefully cut the cut piece with the sharp end into a 2 ml vial