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目的:研究先天性心脏病儿童牙菌斑中白色念珠菌的携带情况。方法:根据WHO龋齿诊断标准,以115名2~5岁的先心病儿童作为研究对象,其中有龋组86人,无龋组29人,同时选取115例健康儿童为对照组,临床检查记录龋失补牙数(dmft)、龋失补牙面数(dmfs)及可视菌斑指数(VPI);采集龈上菌斑及龋坏组织,应用SYBR green I荧光定量PCR方法,对各组样本中白色念珠菌含量进行检测,所获得的数据进行统计学分析。结果:先心病组患龋率为74.8%,dmft、dmfs及VPI分别为5.69±5.69、11.83±15.14、43.8%±24.1%;对照组受检儿童患龋率为67.0%,dmft、dmfs、VPI分别为3.53±4.02、5.51±7.40、36.5%±24.1%。两组dmft,dmfs,VPI的差异有统计学意义。儿童牙菌斑中白色念珠菌的含量(Log10 copies/mL),先心病有龋组龋洞和牙颈部分别为6.71±2.92、5.64±3.11,无龋组为5.03±2.74;正常儿童有龋组龋洞和牙颈部分别为4.01±2.35、3.27±2.14,无龋组为3.43±2.56。先心病儿童与正常儿童牙菌斑中白色念珠菌含量差异具有统计学意义。结论:先心病儿童牙菌斑中白色念珠菌的含量高于正常儿童。口腔中的白色念珠菌,可能成为感染性心内膜炎的菌源之一,使先心病儿童成为感染心内膜炎的易感人群。
Objective: To study the carriage of Candida albicans in dental plaque of children with congenital heart disease. Methods: According to WHO diagnostic criteria of dental caries, 115 children aged 2 to 5 years old with congenital heart disease were enrolled. Among them, there were 86 dental caries patients and 29 caries-free patients, and 115 healthy children were selected as the control group. Clinical examination recorded caries Dmft, dmfs and VPI were measured. The samples of supragingival plaque and carious lesions were collected and quantified by SYBR green I fluorescence quantitative PCR Candida content testing, the data obtained for statistical analysis. Results: The prevalence of caries was 74.8%, dmft, dmfs and VPI were 5.69 ± 5.69, 11.83 ± 15.14 and 43.8% ± 24.1% respectively in the CHD group and 67.0%, dmft, dmfs and VPI Respectively 3.53 ± 4.02,5.51 ± 7.40,36.5% ± 24.1%. The differences of dmft, dmfs and VPI between the two groups were statistically significant. The content of Candida albicans (Log10 copies / mL) in children with plaque was 6.71 ± 2.92,5.64 ± 3.11 in dental caries group and 5.03 ± 2.74 in caries-free group respectively. The normal children had caries Group caries and dental neck were 4.01 ± 2.35,3.27 ± 2.14, caries-free group was 3.43 ± 2.56. There was significant difference in the content of Candida albicans in the dental plaque of children with congenital heart disease and normal children. Conclusion: The content of Candida albicans in dental plaque of children with congenital heart disease is higher than that of normal children. Candida albicans in the mouth, may become one of the sources of infective endocarditis, so that children with congenital heart disease become susceptible to endocarditis infection.