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目的:建立苗药矮地茶的HPLC指纹图谱分析方法。方法:采用HPLC法,Diamonsil C18柱(250 mm×4.6 mm,5μm),甲醇-0.1%磷酸溶液梯度洗脱,流速为1.0 m L/min,柱温35℃,检测波长为250 nm。结果:对10批贵州不同产地样品进行了分析,确定了32个共有峰,构成了矮地茶的HPLC指纹图谱,其中4个共有峰得到指认,分别为没食子酸、岩白菜素、绿原酸、槲皮苷。结论:此方法简便,准确,重复性好,可用于矮地茶的HPLC指纹图谱测定,为今后规范其药材资源及质量评价提供科学依据。
Objective: To establish a method of HPLC fingerprinting analysis of Miao medicine short-tea. Methods: The HPLC method was used on a Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution of methanol-0.1% phosphoric acid solution at a flow rate of 1.0 mL / min and a column temperature of 35 ° C. The detection wavelength was 250 nm. Results: Ten batches of samples from different areas of Guizhou were analyzed and 32 common peaks were identified. HPLC fingerprinting of Dikeicha was carried out. Among them, four common peaks were identified, including gallic acid, bergenin, chlorogenic acid , Quercitrin. Conclusion: This method is simple, accurate and reproducible. It can be used for the HPLC fingerprint determination of Dwarf tea and provide a scientific basis for the future standardization of medicinal resources and quality evaluation.