论文部分内容阅读
应用抗受体相关蛋白羧基端１０８个氨基酸多肽单克隆抗体Ｆ２（ＭｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙＦ２，ＭｃＡｂＦ２）进行被动型Ｈｅｙｍａｎｎ肾炎（ＰａｓｓｉｖｅＨｅｙｍａｎｎｎｅｐｈｒｉｔｉｓ，ＰＨＮ）诱导试验。Ｗｅｓｔｅｒｎ－Ｂｌｏｔ分析显示，ＭｃＡｂＦ２识别天然膜抗原Ｆｘ１Ａ中分子量为４４ＫＤａ的受体相关蛋白（Ｒｅｃｅｐｔｏｒ－ａｓｏｃｉａｔｅｄｐｒｏｔｅｉｎ，ＲＡＰ），ＳＤ大鼠体内注射ＭｃＡｂＦ２４天后肾皮质免疫组化和免疫电镜发现ＭｃＡｂＦ２在体内能识别肾小球上皮细胞表面抗原，并与之结合形成典型的ＰＨＮ免疫复合物。表明在ＲＡＰ羧基端１０８个氨基酸多肽上存在着Ｈｅｙｍａｎｎ肾炎病理性表型。
The Passive Heymann Nephritis (PHN) induction test was carried out using Monoclonalantibody F2 (McAb F2), a carboxyterminal anti-receptor related protein. Western-Blot analysis showed that McAbF2 recognized a 44 kDa Receptor-asociated protein (RAP) in the Fx1A native membrane antigen. Immunohistochemistry and immunoelectron microscopy showed that McAb F2 could recognize in vivo in vivo after McAb F was injected into SD rats Glomerular epithelial cell surface antigen, and combined with the formation of a typical PHN immune complexes. Suggesting a pathological phenotype of Heymann nephritis on the 108 amino acid polypeptide of the carboxy terminal RAP.