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目的:建立高效液相色谱法测定北五味子油水溶液灌胃后大鼠血浆中五味子甲素与五味子乙素的含量,并研究其在大鼠血浆中的药动学行为。方法:采用KromasilC18柱(4.6mm×250mm,5μm);流动相:乙腈-水(80:20)为流动相,流速:1.0mL.min-1;检测波长:254nm;柱温:30℃。以黄体酮为内标,乙腈沉淀蛋白后,测定大鼠血浆中五味子甲素与五味子乙素的含量并计算药动学参数。结果:大鼠血浆样品中,五味子甲素与五味子乙素的线性范围分别为30.5～960.5μg.L-1和42.6～1330.0μg.L-1,日内、日间精密度均<13%。Cmax为(567.6±129.8)μg.L-1和(714.4±335.9)μg.L-1,tmax为(5.33±0.18)h和(5.45±0.21)h,t1/2为(4.4±1.7)h和(5.3±1.8)h。结论:该法准确快速,灵敏度高,可作为大鼠血浆中五味子甲素与五味子乙素的同时检测及其体内药动学研究。
OBJECTIVE: To establish a HPLC method for the determination of Schisandrin A and Schisandrin B in plasma of rats after intragastric administration of aqueous solution of Schisandra chinensis and to study its pharmacokinetics in rat plasma. Methods: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-water (80:20) as mobile phase and the flow rate was 1.0 mL.min-1. The detection wavelength was 254 nm. To progesterone as an internal standard, acetonitrile precipitation protein, determination of rat plasma schisandrin A and schisandrin B content and calculate the pharmacokinetic parameters. Results: The linear range of Schisandrin A and Schisandrin B was 30.5-960.5μg.L-1 and 42.6-1330.0μg.L-1, respectively. The intra-and inter-day precision was less than 13%. The Cmax was (567.6 ± 129.8) μg.L-1 and (714.4 ± 335.9) μg.L-1 respectively, the tmax was (5.33 ± 0.18) h and (5.45 ± 0.21) h, and the t1 / 2 was (4.4 ± 1.7) h And (5.3 ± 1.8) h. Conclusion: The method is accurate, rapid and sensitive. It can be used as a simultaneous detection and in vivo pharmacokinetic study of schisandrin and schisandrin in rat plasma.