以埃坡霉素产生菌粘细菌纤维堆囊菌为出发菌株,经亚硝基胍诱变处理后,在含有前体丙酸盐的平板中筛选抗性突变株,得到一株高产突变株N-19,埃坡霉素产量为18.5 mg/L,比出发菌株提高了69.7%。通过优化发酵培养基的碳、氮源组成,埃坡霉素产量达36.1 mg/L,此时埃坡霉素B和埃坡霉素A的比值为0.4;在摇瓶发酵过程中补加前体丙酸盐,埃坡霉素B和埃坡霉素A的比值增至2.1。 A strain of epothilone-producing myxobacteria was used as starting strain. After mutagenized with nitrosoguanidine, the resistant mutant strains were screened in the plates containing the precursor propionate to obtain a mutant strain N -19 and epothilone 18.5 mg / L, which is 69.7% more than the original strain. By optimizing the carbon and nitrogen sources of the fermentation medium, the yield of epothilone reached 36.1 mg / L, at which time the ratio of epothilone B to epothilone A was 0.4; before the fermentation in shake flask, Propionate, epothilone B and epothilone A ratio increased to 2.1.