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目的 :观察瘤体直接注射 IL-2重组腺病毒对 G4 2 2皮下荷瘤的治疗作用 ,对体内基因转染效率及治疗的免疫机制进行初步分析。方法 :将 L ac Z、IL -2重组腺病毒直接注射到皮下接种的 G4 2 2胶质母细胞瘤瘤体 ,X-gal染色检测基因转染的效率 ,观察肿瘤的大小和荷瘤小鼠的存活期 ,采用 4 h5 1Cr释放法检测荷瘤小鼠脾细胞诱导的 NK、L AK、CTL的杀伤活性 ,对治疗后的肿瘤进行常规病理分析。结果 :采用瘤体注射腺病毒具有较高的体内基因转染效率 ,瘤体注射 IL-2重组腺病毒对皮下建立的胶质母细胞瘤有一定的治疗作用 ,肿瘤的生长受抑制 ,荷瘤小鼠的存活期显著延长 ,但没有长期存活小鼠。IL-2基因治疗组小鼠脾细胞的 LAK、CTL杀伤活性显著增强 ,肿瘤局部出现更多的坏死和炎性浸润细胞。结论 :采用瘤体直接注射 IL -2重组腺病毒对胶质母细胞瘤皮下荷瘤有治疗作用 ,其机制可能是增强了宿主的局部和全身抗肿瘤免疫反应。
Objective: To observe the therapeutic effect of direct injection of IL-2 recombinant adenovirus on G4 2 subcutaneous tumor and to analyze the in vivo gene transfection efficiency and the immune mechanism of treatment. METHODS: Lac Z and IL-2 recombinant adenoviruses were directly injected into subcutaneously inoculated G4 2 glioblastoma tumors. X-gal staining was used to detect the efficiency of gene transfection. Tumor size and tumor-bearing mice were observed. During the survival period, the killing activity of NK, L AK, and CTL induced by spleen cells of tumor-bearing mice was detected by 4 h5 1Cr release method. Routine pathological analysis was performed on the treated tumors. RESULTS: The injection of adenovirus into the tumor had higher in vivo gene transfection efficiency. The injection of IL-2 recombinant adenovirus into the tumor had a certain therapeutic effect on the subcutaneously established glioblastoma. The growth of the tumor was inhibited and tumors were bearing. The survival of mice is significantly longer but there are no long-term surviving mice. The killing activity of LAK and CTL in spleen cells of IL-2 gene-treated mice was significantly increased, and more necrotic and inflammatory infiltrating cells appeared in tumors. Conclusion : Direct injection of IL-2 recombinant adenovirus into tumors has a therapeutic effect on glioblastoma subcutaneous tumors. The mechanism may be to enhance the local and systemic anti-tumor immune responses of the host.