本文介绍了薄层层析扫描的原理、方法及发展概况,应用于中草药含量测定方面按可见光、紫外、荧光三类分别介绍其特点及适用范围,并以实例说明实验精确度及注意事项。本文着重推荐日本平贺敬夫研究的薄层层析扫描对中药制剂的鉴定及质量评价,并论述了应用此法可以在中药制剂的研究及质量监督上起巨大的作用。 This article introduced the principles, methods and development of thin-layer chromatography scanning. It was applied to the determination of Chinese herbal medicine in terms of visible light, ultraviolet, and fluorescence, respectively, to introduce its characteristics and application scope, and to illustrate the experimental accuracy and precautions. This article focuses on the evaluation of the TLC scans by Tsinga Johoff of Japan for the identification and quality evaluation of TCM preparations, and discusses that this method can play a huge role in the research and quality supervision of TCM preparations.