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目的:探讨核因子-κB(NF-κB)活化在肾毒血清性肾炎发病机制中的作用。方法:肾毒血清肾炎应用兔抗鼠肾小球基底膜肾毒血清制备。应用凝胶电泳迁移率检测肾组织中NF-κB活化;采用免疫组化及彩色病理图文分析系统观察肾小球及肾小管中单核细胞趋化蛋白-1(MCP-1)表达,并分析其与蛋白尿和肾小球细胞数之间的关系。结果:模型组肾组织中NF-κB活化较正常对照组显著上调;肾小球及肾小管中MCP-1表达分别为(24.37±7.06)个/肾小球横切面(gcs)和(54.78±11.49)%,较正常对照组显著升高(P<0.01),肾组织中NF-κB活化和MCP-1表达与单核细胞浸润和蛋白尿密切相关。结论:NF-κB活化在肾小球肾炎发病机制中发挥重要作用。
Objective: To investigate the role of nuclear factor-κB (NF-κB) activation in the pathogenesis of nephrotoxic serum nephritis. Methods: Nephrotoxic serum nephritis rabbit anti-rat glomerular basement membrane nephritis serum preparation. The activation of NF-κB in renal tissues was detected by gel electrophoretic mobility shift assay. The expression of MCP-1 in glomeruli and tubules was detected by immunohistochemistry and color histogram analysis system Analysis of its relationship with proteinuria and glomerular cell number. Results: The expression of MCP-1 in glomerulus and tubule was (24.37 ± 7.06) / glomerular cross-section (gcs) and (54.78 ± 11.49)%, which was significantly higher than that of the normal control group (P <0.01). The activation of NF-κB and the expression of MCP-1 in renal tissue were closely related to mononuclear cell infiltration and proteinuria. Conclusion: NF-κB activation plays an important role in the pathogenesis of glomerulonephritis.