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目的调查了解广州及周边地区蝙蝠携带狂犬病毒和禽流感病毒的情况,分析蝙蝠与人类相关疾病的关系。方法2004年9月~2005年11月,在广州市及广州市野生动物市场收集来自广州及周边地区的分属2个亚目7个种的蝙蝠共794只:犬蝠(Cynopterus sphinx)330只、普氏蹄蝠(Hipposideros pratti)7只、棕果蝠(Rousettusleschenault)154只、普通长翼蝠(Miniopterus schreibersi)5只、小黄蝠(Scotophilus kuhlii)52只、中菊头蝠(Rhinolophusaffinis)209只及小菊头蝠(Rhinolophus pusillus)37只。收集的蝙蝠分别取得标本:咽拭713份、血清452份、脑组织742份、肺组织742份及直肠组织742份共计3 391份,进行RT-PCR和细胞分离培养、检测狂犬病毒(RV)与禽流感病毒(AIV)。结果收集的蝙蝠标本均未检测、分离到狂犬病毒和禽流感病毒。结论广州及周边地区的7种蝙蝠可能未携带狂犬病毒和禽流感病毒。
Objective To investigate the bat-borne rabies virus and bird flu virus in Guangzhou and the surrounding areas and analyze the relationship between bat and human diseases. METHODS: From September 2004 to November 2005, 794 bats belonging to 2 subfamilies, 7 species belonging to 2 subfamilies, were collected from Guangzhou and the surrounding areas in Guangzhou and Guangzhou wildlife markets: 330 Cynopterus sphinx 7 Hipposideros pratti, 154 Rousettusleschenault, 5 Miniopterus schreibersi, 52 Phyphiophilus kuhlii, 209 Rhinolophus affinis Only 37 species of Rhinolophus pusillus were found. The collected bats were collected from 713 swab swabs, 452 serum swabs, 742 brain tissues, 742 lung tissues and 742 rectal tissues for a total of 3 391 samples. RT-PCR and cell isolation were used to detect rabies virus (RV) And bird flu virus (AIV). Results The bat specimens collected were not detected, rabies virus and bird flu virus were isolated. Conclusion Seven species of bats in Guangzhou and surrounding areas may not carry rabies virus and bird flu virus.