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目的:探讨从腹腔游离癌细胞中分离胃癌干细胞的可行性及其意义。方法:随机选择35例胃癌患者,术中发现有腹膜转移5例,无腹膜转移30例。用流式细胞仪检测腹腔灌洗液中CD44和CK20的表达,并比较有、无腹膜转移两组之间差别是否有差异。结果:⑴有、无腹膜转移组之间腹腔游离癌细胞中CD44+CK20+差异有统计学意义(P=0.009);⑵CD44在胃癌组织中的阳性表达率为36.8%,胃癌组织中CD44阳性表达率与浆膜浸润、淋巴结转移等病理因素有关;该两因素分组间差异有统计学意义(P=0.000)。结论:从胃癌腹腔灌洗液游离癌细胞中分离胃癌干细胞是可行的,这种可行性为人胃癌干细胞的分离提供了新的思路,为制定胃癌腹膜转移的靶向治疗提供了一定的实验基础。
Objective: To investigate the feasibility and significance of separating gastric cancer stem cells from peritoneal free cancer cells. Methods: 35 cases of gastric cancer were randomly selected. There were 5 cases of peritoneal metastasis and 30 cases of peritoneal metastasis. Flow cytometry was used to detect the expression of CD44 and CK20 in peritoneal lavage fluid and to compare whether there was any difference between the two groups without peritoneal metastasis. Results: (1) CD44 + CK20 + in peritoneal free cancerous cells with or without peritoneal metastasis was significantly different (P = 0.009); (2) The positive expression rate of CD44 in gastric carcinoma was 36.8%, the positive rate of CD44 in gastric carcinoma And serosa invasion, lymph node metastasis and other pathological factors; the two factors were statistically significant differences (P = 0.000). Conclusion: It is feasible to separate gastric cancer stem cells from free cancer cells in peritoneal lavage fluid of gastric cancer. This feasibility provides a new idea for the separation of human gastric cancer stem cells and provides some experimental basis for the targeted therapy of peritoneal metastasis of gastric cancer.