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研究pgm、fba、gap过表达对植物乳杆菌(Lactobacillus plantarum)LR-1的抗逆性、黏附和生物被膜形成的影响.结果 表明,pgm、fba基因的过表达可提高植物乳杆菌LR-1的耐热性、耐酸性和抗胆盐性,这两个基因的过表达促进了该菌株生物被膜的形成和黏附.gap基因的转录水平对植物乳杆菌LR-1的抗逆性、生物被膜形成和黏附没有显著影响.pgm、fba、gap的上调提高了该菌株tuf、 luxS的转录水平.这种增加在过表达pgm、fba的重组菌株中比在过表达ap的重组菌株中高.此外,生物信息学分析表明,pgm、 fba可与多条途径相关的基因相关联.本研究揭示糖酵解途径相关基因在调节植物乳杆菌的抗逆性、黏附和生物被膜形成中的新作用.“,”This study aimed to investigate whether and how pgm,fba,and gap overexpression can affect the stress resistance,adhesiveness,and biofilm formation of Lactobacillus plantarum LR-1.Results showed that the overexpression of the pgm and fba genes enhanced the heat,acid and bile salt resistance,biofilm formation and adhesiveness of L.plantarum LR-1.The transcriptional level of the gap gene had no significant effect on the stress resistance,biofilm formation or adhesiveness.The up-regulation of pgm,fba,and gap increased the transcriptional levels of tuf and luxS in this strain with the first two being more effective than the last one.Furthermore,bioinformatic analyses suggested that pgm and fba could be associated with genes related to multiple pathways.These results provide a new understanding of the role of glycolytic pathway-related genes in regulating the stress resistance,adhesiveness,and biofilm formation of L.plantarum.