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分别以轻、中和重稀土Pr、Eu、Gd、Dy、Lu为中心离子,以桂皮酸(Hcin)和邻菲啰啉(phen)为配体,合成了五种三元稀土配合物。经C、H、N元素分析和稀土络合滴定推测三元配合物的组成为REphen(cin)3(RE3+=Pr3+、Eu3+、Gd3+、Dy3+、Lu3+);红外光谱和紫外吸收光谱测试表明配合物中RE3+与桂皮酸的羧基氧原子及邻菲啰啉的两个氮原子配位成键。采用紫外吸收光谱法及粘度法研究了三元稀土配合物与小牛胸腺DNA(CT-DNA)的作用,结果表明配合物是以插入方式与DNA结合,配合物中的平面分子phen在一定程度上插入到了DNA的碱基对中,与DNA的键合常数随稀土原子序数增大而增大,表明离子半径小的重稀土配合物更容易插入到DNA的碱基对中。
Five kinds of ternary rare earth complexes were synthesized by using Pr, Eu, Gd, Dy and Lu as neutral ions in light, medium and heavy rare earths, and using cinnamic acid and phenanthroline as ligands. The composition of the ternary complex was REphen (cin) 3 (RE3 + = Pr3 +, Eu3 +, Gd3 +, Dy3 +, Lu3 +) by C, H, N elemental analysis and rare earth complexometric titration. The results of IR and UV- In RE3 + and cinnamic acid carboxyl oxygen atoms and two phenanthroline nitrogen atoms coordinate to form a bond. The effects of ternary rare earth complexes and calf thymus DNA (CT-DNA) were studied by UV-Vis spectroscopy and viscometry. The results showed that the complexes were bound to DNA by insertion, Inserted into the base pair of DNA, and the DNA binding constant increased with the increase of rare earth atomic number, indicating that the heavy ion radius of the heavy rare earth complexes more easily inserted into the DNA base pairs.