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目的 阐明芫花根乙醇提取物 (EERD)的抗炎活性。方法 EERD对急性炎症的抑制作用采用组胺诱导的小鼠毛细血管通透性增加、角叉菜胶诱导的大鼠足肿胀以及小鼠网状内皮系统 (RES)对异物的吞噬作用进行分析。对慢性炎症的抑制作用以福氏完全佐剂诱导的多发性关节炎和棉球诱导的大鼠肉芽肿进行分析。结果 EERD在剂量为 4 0 m g/ kg时能明显地抑制小鼠毛细血管通透性增加和抑制大鼠足肿胀 ;在剂量为 30 m g/ kg时对 RES的吞噬能力有提升作用、对大鼠肉芽肿和多发性关节炎表现出显著抑制作用。 EERD也能抑制丙二醛(MDA)、前列腺素 E2 (PGE2 )、一氧化氮 (NO)、肿瘤坏死因子 -α (TNF-α)和白细胞介素 - 1β (IL - 1β)的形成 ,增强超氧化物歧化酶 (SOD)和过氧化氢酶 (CAT)的活力并钝化诱导型氮氧化物合酶 (i NOS)的活性。结论 EERD的抗炎活性主要是通过抑制脂质过氧化反应和炎症介质的释放、增强 SOD和 CAT的活力、钝化 i NOS的活性以及提升 RES的吞噬作用实现的。
Objective To elucidate the anti-inflammatory activity of Ethanol Extract (EERD). Methods The inhibitory effect of EERD on acute inflammation was evaluated by histamine-induced increases in capillary permeability in mice, carrageenan-induced paw swelling in rats, and phagocytosis of foreign bodies by mouse reticuloendothelial system (RES). The inhibitory effect on chronic inflammation was analyzed with Freund’s complete adjuvant-induced polyarthritis and cotton ball-induced rat granuloma. Results EERD at a dose of 40 mg/kg significantly inhibited the increase of capillary permeability in mice and inhibited paw swelling in rats; at a dose of 30 mg/kg, EERD increased the phagocytic ability of RES in rats. Granuloma and polyarthritis showed significant inhibition. EERD also inhibits the formation of malondialdehyde (MDA), prostaglandin E2 (PGE2), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) and enhances The activity of superoxide dismutase (SOD) and catalase (CAT) and passivation of inducible nitric oxide synthase (iNOS) activity. Conclusion The anti-inflammatory activity of EERD is mainly achieved by inhibiting lipid peroxidation and the release of inflammatory mediators, enhancing the activity of SOD and CAT, inactivating iNOS activity, and increasing phagocytosis of RES.