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目的探讨虎红平板凝集试(RBPT)检测人血浆中布氏菌抗体干扰来源及处理对策。方法选取14例布病抗体阳性人员和20例健康人员,收集血清和枸橼酸钠、K2EDTA、肝素钠抗凝血浆,分别做RBPT和试管凝集试验(SAT)分析干扰情况;20例健康人员血清中分别加入3种不同抗凝剂后做RBPT分析是否抗凝剂直接干扰;20例健康人员3种不同抗凝剂血浆加入凝血酶时间(TT)检测试剂,温育1 h后,取上清做RBPT分析干扰来源和应对措施。结果 14例布病抗体阳性血清和血浆SAT结果相同;20例健康人员血清和血浆的RBPT结果完全相反,血清中加入3种不同抗凝剂对RBPT无影响,经TT试剂去除纤维蛋白原处理后的血浆标本除肝素钠组外,其他RBPT结果均与血清组相同。结论血浆不能直接做RBPT,纤维蛋白原能与虎红抗原产生非特异性凝集,是RBPT干扰的来源;可以通过TT试剂去除纤维蛋白原后消除干扰;在无法得到血清标本时,也可以直接用血浆做SAT来提供诊断参考。
Objective To investigate the source of tiger plate agglutination test (RBPT) in human plasma and the treatment countermeasures. Methods 14 cases of brucellosis-positive patients and 20 healthy persons were selected. Serum and sodium citrate, K2EDTA and heparin sodium anticoagulated plasma were collected. RBPT and test tube agglutination test (SAT) were used to analyze the interference. 20 healthy volunteers Were added three different anticoagulants RBPT analysis whether anticoagulant direct interference; 20 healthy people three different anticoagulant plasma added thrombin time (TT) detection reagent, incubated for 1 h, the supernatant Do RBPT analysis of sources of interference and countermeasures. Results The results of 14 cases of brucine antibody-positive serum and plasma SAT were the same. The RBPT results of serum and plasma of 20 healthy people were completely opposite. There were no effects on the RBPT after adding 3 kinds of different anticoagulants in serum. After removing fibrinogen by TT reagent Of the plasma samples except for the heparin sodium group, other RBPT results were the same as the serum group. Conclusion The plasma can not be directly RBPT, fibrinogen and tiger red antigen non-specific agglutination, is a source of RBPT interference; can be removed by TT reagent fibrinogen to eliminate interference; can not be obtained in serum samples can also be directly used plasma SAT to provide a diagnostic reference.