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在东北林业大学育种基地,利用9种浓度(0.3%、06%、0.9%、1.2%、1.5%、1.8%、2.1%、2.4%、3.0%)的Na2CO3和NaHCO3混合液对白榆扦插苗进行胁迫处理,探讨其在盐胁迫下,细胞质膜的伤害程度(电解质的相对外渗率)和质膜保护系统相关酶的活性(超氧物歧化酶)。结果表明,白榆在受到不同浓度的盐胁迫下,其叶片的电解质相对外渗率发生明显的变化。在一定盐浓度范围内叶片的电解质相对外渗率上升的趋势比较缓慢,表明白榆的细胞质膜对盐离子的伤害有较强的抗性。当土壤基质盐浓度大于1.5%时,叶片的电解质相对外渗率显著增加。白榆叶片中SOD的活性在盐浓度低于1.5%时呈现一个上升的趋势,苗木的生长正常,没有受伤害的症状,但是盐浓度超过1.5%时,SOD的活性快速下降,电解质相对外渗率迅速提高。
In the breeding base of Northeast Forestry University, cuttings of Ulmus pumila were treated with a mixture of Na2CO3 and NaHCO3 at 9 concentrations (0.3%, 06%, 0.9%, 1.2%, 1.5%, 1.8%, 2.1%, 2.4%, 3.0% Stress treatment, to explore its salt stress, the plasma membrane damage degree (electrolyte relative infiltration rate) and plasma membrane protection system related enzyme activity (superoxide dismutase). The results showed that under the salt stress of Ulmus pumila, the relative electrolyte leakage rate of leaves significantly changed. In a certain salt concentration range, the relative electrolyte leakage rate of leaves increased slowly, indicating that the plasma membrane of Ulmus pumila was more resistant to salt ions injury. When the soil matrix salt concentration is more than 1.5%, the relative electrolyte leakage rate of the leaves is significantly increased. The activity of SOD in Ulmus pumila leaves showed a rising trend when the salt concentration was less than 1.5%. The growth of seedling was normal without any damage. However, when the salt concentration exceeded 1.5%, the activity of SOD decreased rapidly and the electrolyte was extravasated Rate rapidly increased.