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上颈交感神经节具有较完整的神经突触传递的外周神经组织,是研究神经药理学的一个较好的材料。鉴于在体内实验研究,上颈交感神经节虽可保持生理条件,但用于药物定量研究不够理想。1956年Pascol建立离体神经节,用表面浮动电极记录节电位的方法操作过于复杂。1975年Brown改进此方法,采取液体滴灌及固定电极的方法,从而能连续地观察记录药物引起节电位变化的整个过程。对研究神经节药理提供了一个好方法。Brown对此方法介绍非常简单。经过摸索和改进,我们建立了此方法,并进行了一系列实验。材料和方法一、电极的制备 1.Ag/AgCl_2乏极化电极的制备:取银
Upper cervical sympathetic ganglia with more complete synaptic transmission of peripheral nerve tissue, is to study the neuropharmacology of a better material. In view of in vivo experimental studies, although the upper cervical sympathetic ganglia can maintain the physiological conditions, but for the quantitative study of drugs is not ideal. In 1956, Pascol established an in vitro ganglion, and the method of recording power-saving potential with a surface-floating electrode was too complicated. Brown improved this method in 1975 and took a method of drip irrigation and electrode fixation to continuously observe the entire process of recording power-induced potential changes. Provides a good way to study ganglion pharmacology. Brown introduced this method is very simple. After exploration and improvement, we built this method and conducted a series of experiments. Materials and methods First, the preparation of the electrode 1.Ag / AgCl_2 preparation of the polarization electrode: take silver